Dr. Wang-Sik Lee joined the Department of Medicine in the Division of Hematology as an Assistant Professor on January 22, 2019.
After Dr. Lee earned his Ph.D in Biochemistry, he worked at the Korea Atomic Energy Research Institute as a researcher, at the Friedrich Miescher-Institute (Switzerland) as a research fellow, and at the Biotechnology Laboratory in CKD (Chong Kun Dang) Research Institute as a group leader.
He has a special interest in bacterial, insect and mammalian cell culture, DNA manipulation, GST pulling-down assays and Protein expression and purification, including insect proteins and antibodies. He specializes in Affinity purification using abtibodies.
Dr. Lee is an expert in handling HPLC, FPLC, Gas Chromatography, paper chromatography and thin-layer chromatography, Chromatofocusing, Fermentor, and immunofluorescence microscopes. He can perform DNA and Protein Gel Electrophoresis and can purifiy DNA and RNA.
Just before joining Washington University School of Medicine, his work was focused on expressing and purifying recombinant human insulin using E. coli expression systems. The expressed proteins were purified using an industrial scale FPLC system. While conducting this research, Dr. Lee will apply for patents in preparing for recombinant human insulin.
Since Dr. Lee joined Washington University School of Medicine, he has worked on the trafficking of mannose 6-phosphate uncovering enzyme and N-acetyl glucosamine -1- phosphotransferase, which are involved in lysosomal enzymes targeting to lysosomes from 1999 to 2017.